Syddansk Universitet MEMPHYS - Center for Biomembrane Physics
Syddansk Universitet

Homepage of Luis A. Bagatolli

Research interest

Biological membranes are sheet like structures mainly composed of lipids and proteins. These structures are only two (lipid) molecules thick (thickness ~ 60 Å (6 nm) to 100 Å (10 nm) depending on the particular membrane). Such membranes typically define enclosed spaces or compartments in which cells may maintain a chemical or biochemical environment that differs from the outside. All the communication from the inside to the outside of the cell obviously may cross the membranes. Our main research interest is to study the lateral distribution and dynamics of particular molecules (particularly lipids and proteins) in different artificial lipid membrane’s model systems (liposomes and supported bilayers) and particular biological membranes (such as skin stratum corneum lipid membranes and pulmonary surfactant membranes). These last parameters are very important to understand general aspects of membrane function. For example it is well known that changes in the lateral structure of membranes (by changing composition, temperature, pH or ionic strength) may induce changes in the mechanical properties of the membrane that in turn may affect the biochemical/biological response of the membrane. The main techniques used in these studies are based in Fluorescence spectroscopy/microscopy (including confocal fluorescence microscopy, multiphoton excitation fluorescence microscopy and second harmonic generation microscopy). Fluorescence spectroscopy measure the light emitted by particular molecules (fluorescent molecules or probes). In some cases the emitted light (fluorescence) is sensitive to the molecular environment of the probe. In the case of fluorescence microscopy an additional spatial correlation is obtained (fluorescence image). See also "Membrane Biophysics and Biophotonics group" at the Department of Biochemistry and Molecular Biology, SDU. MB&B

Cover gallery

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BBActa-Biomembranes 1798(7):1285-1456 (July 2010), Special issue "Microscopy Imaging of Membrane domains"(L.A. Bagatolli Guest Ed.) view►

Selected References

1) I. Iwai, H. Han, L.D. Hollander, S. Svensson, L.G. Ofverstedt, J. Anwar, J. Brewer, M. Bloksgaard, A. Laloeuf, D. Nosek, S. Masich, L.A. Bagatolli, U. Skoglund and L Norlén. 2012. The Human Skin Barrier Is Organized as Stacked Bilayers of Fully Extended Ceramides with Cholesterol Molecules Associated with the Ceramide Sphingoid Moiety. J Invest Dermatol. doi: 10.1038/jid.2012.43. [Epub ahead of print] view►

2) R.P. Stock, J. Brewer, K. Wagner, B. Ramos-Cerrillo, L. Duelund, K. Drescher Jernshøj, L. Folke Olsen and L. A. Bagatolli. 2012. Sphingomyelinase D Activity in Model Membranes: Structural Effects of in situ Generation of Ceramide-1-Phosphate. PLoS ONE 7(4): e36003. doi:10.1371/journal.pone.0036003 view►

3) P. Husen, M. Fidorra, S. Hartel, L.A. Bagatolli and J.H. Ipsen, 2012. A method for analysis of lipid vesicle domain structure from confocal image data. Eur. Biophys. J. 41(2):161-175. view►

4) J. Kubiak, J.R. Brewer, S. Hansen and L.A. Bagatolli. “Lipid lateral organization on giant unilamellar vesicles containing lipopolysaccharides”, 2011, Biophys. J. 100: 978-986 view►

5) J. Brewer, J. Bernardino de la Serna , K. Wagner and L.A. Bagatolli. “Multiphoton excitation fluorescence microscopy in planar membrane systems”, 2010, Biochim. Biophys. Acta. 1798(7):1301-1308 view►

6) L.A. Bagatolli, J.H. Ipsen, A.C. Simonsen and O.G. Mouritsen “An outlook on organization of lipids in membranes: Searching for a realistic connection with the organization of biological membranes”, 2010 Prog. Lipid Res. view►

7) M. Fidorra, A. Garcia, J. Ipsen, S. Hartel and L.A. Bagatolli “Lipid domains in giant unilamellar vesicles and their correspondence with equilibrium thermodynamic phases: A quantitative fluorescence microscopy imaging approach”, 2009 Biochim. Biophys. Acta 1788(10):2142-2149 view►

8) U. Bernchou, J. R. Brewer, H. S. Midtiby, J. H. Ipsen, L. A. Bagatolli and A. C. Simonsen, “Texture of lipid bilayer domains” 2009, Journal of the American Chemical Society 131(40):14130-1431. view►

9) D.C. Carrer, C.Vermehren and L.A Bagatolli. 2008. “Pig skin structure and transdermal delivery of liposomes: A two photon microscopy study ”. J. Controlled Release, 132(1):12-20. view►

10) L-R Montes, A. Alonso, F. Goñi and L.A Bagatolli. 2007. “Giant unilamellar vesicles electroformed from native membranes and organic lipid mixtures under physiological conditions ”. Biophys. J 93(10):3548-3554. view►

11) Plasencia-Gil, L. Norlen,and L.A: Bagatolli. 2007 “Direct visualization of lipid domains in human skin stratum corneum’s lipid membranes: effect of pH and temperature” Biophys. J 93(9):3142-3155view►

12) Bagatolli L.A. 2006. “To see or not to see: lateral organization of biological membranes and fluorescence microscopy” Review article. Biochim Biophys Acta 1758:1541-1556.view►

13) Bernardino de la Serna J., J. Perez-Gil, A. C. Simonsen and L.A. Bagatolli. 2004. “Cholesterol rules: direct observation of the coexistence of two fluid phases in native pulmonary surfactant membranes at physiological temperatures” J. Biol. Chem. 279:40715-40722.view►

14) Dietrich C., L. A. Bagatolli, Z. Volovyk, N. L. Thompson, M. Levi, K. Jacobson and E. Gratton. 2001. “Lipid Rafts Reconstituted in Model Membranes”. Biophys. J. 80: 1417-1428.view►

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Last modified: 09/09/2013